isolation and detection of virulence factors of salmonella typhimurium and salmonella enteritidis
نویسندگان
چکیده
plasmids play an important role in the virulence and antibiotic resistance of salmonella. salmonella carry plasmids having different size and number. plasmid profile analysis of single isolates of salmonella typhimurium and salmonella enteritidis revealed the presence of 4- plasmid profiles. salmonella serovars produce several type iii secretions including sop-e and sop –b. soap e is detected by dot-elisa. dot-elisa is one of the solid phase immuneassay used to detect either antigens or antibody. sop-b (salmonella outer protein-b) is detected by multiplex pcr method using pcr primers sop p1 and sop- m13. it is concluded that the technique used in this study is more sensitive and identification and determination of various molecular characteristics of two species enhances the knowledge towards the vaccine production.
منابع مشابه
Isolation and Detection of Virulence factors of Salmonella typhimurium and Salmonella enteritidis
Plasmids play an important role in the virulence and antibiotic resistance of Salmonella. Salmonella carry plasmids having different size and number. Plasmid profile analysis of single isolates of Salmonella typhimurium and Salmonella enteritidis revealed the presence of 4- plasmid profiles. Salmonella serovars produce several Type III secretions including Sop-E and Sop –B. Soap E is detected b...
متن کاملIsolation and Detection of Virulence factors of Salmonella typhimurium and Salmonella enteritidis
ARTICLE INFO ABSTRACT Article history: Received 17 June 2012 Accepted 3 September 2012 Available online 1 November 2012 Plasmids play an important role in the virulence and antibiotic resistance of Salmonella. Salmonella carry plasmids having different size and number. Plasmid profile analysis of single isolates of Salmonella typhimurium and Salmonella enteritidis revealed the presence of 4plas...
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عنوان ژورنال:
international journal of molecular and clinical microbiologyجلد ۲، شماره ۲، صفحات ۱۶۴-۱۶۹
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